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Operetta CLS High-Content Analysis System

Uncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS™ high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony 4.5 software, Operetta CLS™ lets you find even subtle phenotypic changes.

Description

The Operetta CLS system combines speed and sensitivity with the powerful and intuitive data analysis you’ve come to trust from the Operetta platform. The all-new Operetta CLS delivers everything you need from high-content analysis. What’s more, the Operetta CLS system is part of our comprehensive HCS workflow – everything from HCS systems and microplates to automation and informatics for every application. All from one knowledgeable, trusted vendor. Put that together with our Harmony® high-content imaging and analysis software – the easy-to-learn, easy-to-use software that empowers biologists to do their own analysis – and you have everything you need to run your everyday (and complex) analyses right away.


A Unique Combination of Technologies


At the core of the Operetta CLS high-content analysis system is a new light path that ensures efficient excitation of your samples and careful collection of emitted signals.


  • Powerful, stable 8x LED light source for optimal excitation of your fluorophores and confidence in results
  • Proprietary automated water-immersion objectives with very high numerical aperture enable high resolution and fast read times with minimal photodamage
  • Confocal spinning disk technology provides a fast and gentle imaging process, enabling efficient background rejection, live cell experiments, and 3D imaging
  • Large format sCMOS camera delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times
  • User accessible bar-coded emission filters provide flexibility, sensitive imaging and error-free operation
  • Simple, powerful Harmony 4.5 high-content imaging and analysis software for easy set-up of assays, automation of experiments, image acquisition, data analysis, and storage and retrieval of results

A Solution Configured To Suit Every Need;


Whatever your application, there’s an Operetta CLS system configured to meet your requirements. And it’s modular, so it can change with your research demands. Several configuration options are available, Typical configurations include:


Operetta CLS Quattro


  • With four LEDs and widefield fluorescence, the basic configuration of the Operetta CLS system is ideal for common applications that need sensitivity and resolution, with the capacity to grow if the need arises.

Operetta CLS FLEX


  • With eight LEDs, and confocal and widefield fluorescence, the FLEX configuration offers flexibility in excitation and imaging modes for many challenging applications – and it can be upgraded to even higher performance.

Operetta CLS LIVE


  • With all the features of the FLEX configuration, plus gas and temperature control and water-immersion objectives, this system is ideal for gentle yet highly sensitive live cell imaging.

Applications


From everyday assays to more demanding applications, the Operetta CLS high-content analysis system delivers just the right combination of flexible excitation, sensitive optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.


Fixed-cell assays


  • Up to eight high-power excitation sources and user-accessible emission filters enable maximum flexibility of fluorescent stains and labels, plus the system features widefield and spinning-disk fluorescent imaging.

Live-cell assays


  • The spinning disk confocal optics and synchronized LED illumination provide stable excitation and minimize phototoxicity and bleaching for meaningful live-cell assays. You can also choose brightfield or digital-phase contrast imaging modes.

Complex cellular models


  • The large-format sCMOS camera with water-immersion objectives provides sensitivity and high resolution, while advanced software helps you address the imaging and analysis challenges presented by complex cellular models.

Advanced assays


  • FRET is a powerful tool for investigating conformational changes and protein-protein interactions. The Operetta CLS system’s sensitive imaging and dedicated analysis tools for ratiometric imaging, facilitate robust results.

Phenotypic fingerprinting


  • The Operetta CLS combines high resolution imaging with advanced software tools to help create robust phenotypic fingerprints of the subtle differences at the core of successful phenotypic assays.
Learn more

Specifications

Contact

Nova Natura UAB

Kaštonų g. 56, Giraitės k, LT-54310,
Kaunas, Lithuania

nova@novabio.lt
Phone: +370 (620) 70159

Reg No 305063819

Nova Natura OÜ

Valukoja 8, 11415,
Tallinn, Estonia

nova@novabio.ee
Phone: +372 5656 0050

Reg No 14758203

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