5 - 20 ml
Ultrafiltration unit with unique twin membrane for sample concentration with high process flexibility: centrifugation or centrifugation of pressurized units, as well as pressure filtration in stationary benchtop mode.
Ultrafiltration unit with a MWCO of 10,000 Dalton and a unique twin membrane (polyethersulfone), suitable for starting volumes up to 20 ml for sample concentration with high process flexibility: centrifugation or centrifugation of pressurised units, as well as pressure filtration in stationary benchtop mode.
Vivaspin® 20 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin® 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin® 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin® 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin® 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. 'Pressure-fugation' is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.
· High flux PES membrane
· High retentate recovery > 90 %
· Vertical twin membrane for ultra fast concentration
· Universal rotor compatibility
· Safe sample concentration due to dead-stop volume
· Convenient concentrate recovery by pipetting
· Volume graduation for easy reading/monitoring
· 100-fold concentration with twin vertical membranes
· Additional option for pressurized operation
Vivaspin® 20 Polyethersulfone, 10 k Da
Rotor Compatibility
Please note: Vivaspin® 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin® 15, which can be used in either round bottom or conical centrifuge tubes
