PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.
PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.
PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 540nm. It is commonly used to stain cells' nuclear compartment.
PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Features
| Numbers of Vials Per Unit | 1 |
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| Quantity or Volume Per Vial | 10mg (10 mg/mL - 16.23mM) |
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| Form | Liquid |
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| Storage | -20°C |
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| Recommended Working Concentration | 2µg/mL (3.24µM) |
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| Maximum Excitation Wavelength | 357 nm |
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| Maximum Emission Wavelength | 540 nm |
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| Common filter set | DAPI |
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| Live Cell Staining | Yes |
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| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
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| Equivalent Number of Microplates | 170-520 x 96-well microplates
140-520 x 384-well microplates
270-800 x 1536-well microplates |
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For research use only.
