PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.
PhenoVue Nile Red Lipid stain exhibits:
- maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
- maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids
PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.
Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
Features
| Numbers of Vials Per Unit | 2 |
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| Quantity or Volume Per Vial | 10mg (31.4µmoles) |
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| Form | Powder |
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| Storage | -20°C |
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| Recommended Working Concentration | 200nM (0.008ng/mL) |
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| Maximum Excitation Wavelength | 512 nm - complexed with triglycerides
552 nm - complexed with phospholipids*
(*as shown in SpectraViewer below) |
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| Maximum Emission Wavelength | 585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below) |
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| Common Filter Set | Cy3.5 |
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| Live Cell Staining | Yes |
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| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
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| Equivalent Number of Microplates | 10900-32700 x 96-well microplates
9090-32700 x 384-well microplates
17040-40900 x 1536-well microplates |
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For research use only.
