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Testing LNP encapsulation efficiency (EE%) is now easier

rugs. 30, 2025

5 min

Conquer LNP characterization

Stunner AF is the only system that pulls together UV/Vis concentration with rotating angle dynamic light scattering (RADLS) and dye-based fluorescence on the same 2 µL sample. Nail down your lipid nanoparticle quality by knocking size, encapsulation efficiency (EE%), particle concentration and aggregate detection off your list in one hit. Without skipping a beat, you’ll know if your nanoparticle is good to go.

Stunner AF -The Ultimate LNP Characterization Tool | Unchained

The Problem
Lipid nanoparticles (LNPs) are all the rage as delivery vehicles for the next generation of mRNA-based gene therapies, antisense oligos and the raw materials for CRISPR. Testing LNP encapsulation efficiency (EE%) is fundamental for knowing if your LNPs are successfully surrounding your payload after production. Traditional EE% assays are a huge hassle for researchers with complex experimental designs that require tons of pipetting steps, poor reproducibility, low sample throughput, and messy toxic surfactants. Not to mention all that headache only produces a single piece of data: EE%. Tools that level up the EE% assay to make it simpler, faster, safer and deliver more than one datapoint per experiment are needed to push nanoparticle development faster and further.

The Solution
Stunner Add Fluorescence (Stunner AF) revolutionizes the EE% assay by measuring free RNA concentrations by fluorescence and total RNA by UV/Vis while simultaneously capturing sizing, PDI and particle concentration data – all in about two hours and all in a 96 well plate-based format. The assay makes EE% characterization more efficient by simplifying assay setup and eliminating the need for nasty surfactant and only consumes 2 μL of sample per well. If you’re hunting for the optimal formulation, Stunner AF handles up to 44 samples on one plate along with standards to deliver EE%, size, aggregation and particle concentration data in a single assay.

Additional information: nova@novabio.lt

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Nova Natura UAB

Kaštonų g. 56, Giraitės k, LT-54310,
Kaunas, Lithuania

nova@novabio.lt
Phone: +370 (620) 70159

Reg No 305063819

Nova Natura OÜ

Valukoja 8, 11415,
Tallinn, Estonia

nova@novabio.ee
Phone: +372 5656 0050

Reg No 14758203

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